Effect of 6-hour exposure to 20 degrees C on the ATP content and other biochemical measures of CPDA-1 packed red cells.

Author(s): Ecker T, Hitzler WE

Publication: Clin Lab, 2000, Vol. 46, Page 291-3

PubMed ID: 10853239 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of a 6 h exposure to room temperature during or at the end of refrigerated storage of pRBCs at 4 degrees C on markers of pRBC quality. Specimens were collected in citrate phosphate dextrose adenosine solution 1 (CPDA-1). Specimens were warmed for 6 h on day 5, 15 or 30 and then returned to storage for 0, 1, 5, 20 (those warmed on day 5 and 15 only) and 30 days (those warmed on day 5 only). ATP content were normalized to Hb content.

   Summary of Findings:

   The concentrations of ATP and glucose declined, and lactate and potassium increased with refrigerated storage. A 6 h room temperature interruption in refrigerated storage had no effect on lactate, glucose, sodium or potassium levels, but the effects on ATP depended on which day specimens were left at room temperature and the duration of refrigerated storage afterwards. Importantly, regardless of warming or storage duration, ATP levels remained above the commonly used threshold of 2 umol/g Hb for pRBC survival.

   Biospecimens

   • Cell - Red Blood Cells

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Hematology/ auto analyzer
   Protein	Spectrophotometry
   Small molecule	Clinical chemistry/auto analyzer
   Cell count/volume	Hematology/ auto analyzer
   Carbohydrate	Clinical chemistry/auto analyzer
   Electrolyte/Metal	Flame emission photometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	0 h 6 h
   Storage	Storage duration	5 days 6 days 10 days 16 days 25 days 30 days 31 days 35 days

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