Venous and skin puncture blood counts compared.
Author(s): Bellamy GJ, Hinchliffe RF
Publication: Clin Lab Haematol, 1988, Vol. 10, Page 329-34
PubMed ID: 3180699 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
-
Study Purpose
The purpose of this study was to determine the effects of obtaining blood by skin prick rather than venipuncture and sequential collection on hemoglobin, red blood cell counts, hematocrit, neutrophil counts, platelet counts, and white blood cell counts in children with leukemia and other illness and among healthy adults. Skin puncture specimens were obtained about 2 hours prior to venipuncture specimens, and all specimens were collected in EDTA tubes.
Summary of Findings:
For both children and adults, average values for hemoglobin, hematocrit, red blood cell counts, and neutrophil counts were significantly higher in blood specimens obtained by skin prick as compared to those obtained by venipuncture. In addition, platelet counts were insignificantly lower and white blood cell counts were insignificantly higher in skin prick specimens. In the majority of individual cases, there were no clinically important differences in cell counts. Individual hemoglobin values were more variable among the specimens drawn from sick children compared to those from healthy adults. The percentage platelets in skin puncture specimens, relative to venipuncture specimens, was relatively stable between sequentially collected skin puncture specimens.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
- Other diagnoses
- Neoplastic - Leukemia
Platform:
Analyte Technology Platform Cell count/volume Hematology/ auto analyzer Protein Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Method of fluid acquisition Venipuncture
Finger/heel prick sampling
Biospecimen Aliquots and Components Aliquot sequential collection 1st collection
2nd collection
3rd collection
4th collection
5th collection
Preaquisition Patient age Adult
6 months-14 years old
Preaquisition Diagnosis/ patient condition Anemia
Hodgkin's disease
Chest infection
Biospecimen Acquisition Anatomical location of blood draw Capillary
Vein