Transient viremia in HIV-infected patients and use of plasma preparation tubes.

Author(s): Stosor V, Palella FJ Jr, Berzins B, Till M, Leake A, Chmiel JS, Murphy RL

Publication: Clin Infect Dis, 2005, Vol. 41, Page 1671-4

PubMed ID: 16267742 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of using Vacutainer PPT instead of Vacutainer EDTA tubes for collection and storage of plasma on quantification of HIV-1 RNA in specimens from patients with suppressed virus receiving highly active antiretroviral therapy. Plasma from EDTA tubes was transferred to new tubes for storage at -70 degrees C while plasma from PPT was kept in the primary collection tube during storage.

   Summary of Findings:

   Switching to PPT from EDTA tubes for specimen collection and storage resulted in 66.1% of patients who had previously suppressed HIV-1 showing low level viremia. When the use of EDTA tubes for collection resumed, only 5.4% of specimens from patients continued to have quantifiable viral loads.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • AIDS/HIV-related

   Platform:

   Analyte	Technology Platform
   RNA	Real-time qRT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	Vacutainer PPT Vacutainer EDTA tubes

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