Effect of multiple freeze-thaw cycles on detection of measles, mumps, and rubella virus antibodies.
Author(s): Pinsky NA, Huddleston JM, Jacobson RM, Wollan PC, Poland GA
Publication: Clin Diagn Lab Immunol, 2003, Vol. 10, Page 19-21
PubMed ID: 12522034 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to determine the effects of freeze-thaw cycling on levels of measles, mumps and rubella immunoglobulin G (IgG) in serum.
Conclusion of Paper
Measles, mumps, and rubella antibody levels were unaffected when serum specimens were subjected to up to 10 freeze-thaw cycles.
Studies
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Study Purpose
The purpose of this study was to determine the effects of freeze-thaw cycling on levels of measles, mumps and rubella IgG in serum. Specimens were refrigerated at 4 degrees C after cycling until analysis.
Summary of Findings:
Measles, mumps, and rubella antibody levels in serum were unaffected by up to 10 freeze-thaw cycles.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Peptide ELISA Protein ELISA Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Freeze/thaw cycling 0 cycles
2 cycles
4 cycles
6 cycles
8 cycles
10 cycles
Storage Storage temperature 4 degrees C
-80 degrees C
ELISA Specific Targeted peptide/protein IgG for measles
IgG for mumps
IgG for rubella