Reference range of hemolysis index in serum and lithium-heparin plasma measured with two analytical platforms in a population of unselected outpatients.
Author(s): Lippi G, Giavarina D, Gelati M, Salvagno GL
Publication: Clin Chim Acta, 2014, Vol. 429, Page 143-6
PubMed ID: 24362229 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of analyzing serum versus lithium heparin plasma and type of auto analyzer on the measurement of HI. Specimens were obtained from 135 outpatients. The order of draw between the serum and lithium heparin tubes was alternated. Serum and plasma were stored at -70 degrees C for one week before analysis.
Summary of Findings:
When cell free hemoglobin was determined using the Cobas c501, significantly higher levels were measured in serum than in plasma. On the other hand, no significant differences in cell free hemoglobin were measured between serum and plasma using the Siemens Dimension Vista 1500. Cell free hemoglobin values for both plasma and serum were slightly but significantly higher when measured with the Siemens Dimension Vista 1500 than with the Cobas c501 (p<0.01 and p=0.02, respectively).
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Protein Spectrophotometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Spectrophotometry Specific Technology platform Roche Cobas c501
Siemens Dimension Vista 1500
Biospecimen Aliquots and Components Blood and blood products Plasma
Serum