NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Reference range of hemolysis index in serum and lithium-heparin plasma measured with two analytical platforms in a population of unselected outpatients.

Author(s): Lippi G, Giavarina D, Gelati M, Salvagno GL

Publication: Clin Chim Acta, 2014, Vol. 429, Page 143-6

PubMed ID: 24362229 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine the effects of analyzing serum versus lithium heparin plasma and type of auto analyzer on the measurement of hemolysis index (HI).

Conclusion of Paper

Significantly higher cell free hemoglobin levels were measured in serum than in plasma by the Roche Cobas c501 but not by the Siemens Dimension Vista 1500. Cell free hemoglobin values for both plasma and serum were slightly but significantly higher when measured with the Siemens Dimension Vista 1500 than with the Cobas c501.

Studies

  1. Study Purpose

    The purpose of this study was to determine the effects of analyzing serum versus lithium heparin plasma and type of auto analyzer on the measurement of HI. Specimens were obtained from 135 outpatients. The order of draw between the serum and lithium heparin tubes was alternated. Serum and plasma were stored at -70 degrees C for one week before analysis.

    Summary of Findings:

    When cell free hemoglobin was determined using the Cobas c501, significantly higher levels were measured in serum than in plasma. On the other hand, no significant differences in cell free hemoglobin were measured between serum and plasma using the Siemens Dimension Vista 1500. Cell free hemoglobin values for both plasma and serum were slightly but significantly higher when measured with the Siemens Dimension Vista 1500 than with the Cobas c501 (p<0.01 and p=0.02, respectively).

    Biospecimens
    Preservative Types
    • Frozen
    Diagnoses:
    • Not specified
    Platform:
    AnalyteTechnology Platform
    Protein Spectrophotometry
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Spectrophotometry Specific Technology platform Roche Cobas c501
    Siemens Dimension Vista 1500
    Biospecimen Aliquots and Components Blood and blood products Plasma
    Serum

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