Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Variation in clinical vitamin D status by DiaSorin Liaison and LC-MS/MS in the presence of elevated 25-OH vitamin D2.

Author(s): de Koning L, Al-Turkmani MR, Berg AH, Shkreta A, Law T, Kellogg MD

Publication: Clin Chim Acta, 2013, Vol. 415, Page 54-8

PubMed ID: 22975531 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of measuring 25-OH vitamin D levels in plasma by the DiaSorin Liaison assay versus by LC-MS/MS in specimens containing high and low levels of 25-OH vitamin D2. Plasma was stored at 4 degrees C for less than 7 days prior to analysis.

   Summary of Findings:

   Significantly lower 25-OH vitamin D concentrations were measured by the Liaison analyzer than by LC-MS/MS (average of 26 nmol/L less), particularly among specimens with high 25-OH vitamin D2 concentrations (average of 34.1 nmol/L less).

   Biospecimens

   • Bodily Fluid - Plasma

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Small molecule	LC-MS or LC-MS/MS
   Small molecule	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   LC-MS or LC-MS/MS Specific	Technology platform	Liaison analyzer
   Preaquisition	Biomarker level	>25 nmol/L 25-OH vitamin D2 <2.5 mol/L 25-OH vitamin D2

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