The characteristics of remnant lipoproteins in the fasting and postprandial plasma.

Author(s): Nakajima K, Nakano T, Tokita Y, Nagamine T, Yatsuzuka S, Shimomura Y, Tanaka A, Sumino H, Nara M, Machida T, Murakami M

Publication: Clin Chim Acta, 2012, Vol. 413, Page 1077-86

PubMed ID: 22433787 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of timing of collection after an oral fat load on levels of cholesterol, triglycerides, and remnant lipoproteins in serum and plasma from healthy volunteers. The oral fat load consisted of subjects ingesting 17 g/m2 body surface area of fat emulsion OFTT cream.

   Summary of Findings:

   RLP-TG and RLP-C increased significantly in serum and plasma obtained 2 h after an oral fat load and remained significantly increased for as long as 6 h after (last time point measured), compared to specimens obtained before the oral fat load. TG and Apo B-48 were also significantly increased in serum and plasma specimens obtained after the oral fat load, but in general, Apo B-100, Apo C-III, total C, HDL-C, and LDL-C were not. Further, the RLP-TG/TG ratio showed no significant changes in specimens taken after oral fat load compared to the fasting specimens, while the RLP-TG/RLP-C ratio was significantly increased specimens taken after oral fat load compared to the ones prior to oral fat load. Significant correlations were found between fasting and after oral fat load levels of RLP-Apo B-100 and TG (r=0.89 and r=0.91, respectively), fasting levels of RLP-Apo B-48 and TG (r=0.69), fasting and after oral fat load levels of RLP-Apo B-100 and RLP-Apo B-48 (r=0.56 and r=0.33, respectively), fasting and after oral fat load levels of RLP-Apo B-100/RLP-Apo B-48 and TG (r=0.76 and r=0.92, respectively).

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Lipid	Enzyme assay
   Steroid	Enzyme assay
   Lipoprotein	Enzyme assay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Time of biospecimen collection	After 12 h of fasting 1 h after oral fat load 2 h after oral fat load 4 h after oral fat load 6 h after oral fat load
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum

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