Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Effects of temperature and light on the stability of bilirubin in plasma samples.

Author(s): Sofronescu AG, Loebs T, Zhu Y

Publication: Clin Chim Acta, 2012, Vol. 413, Page 463-6

PubMed ID: 22108388 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage temperature and light exposure on bilirubin levels in plasma.

   Summary of Findings:

   Bilirubin concentrations were not significantly affected by storage of light protected plasma at 3 or 22 degrees C for 24 h. Specimens with normal or elevated bilirubin levels were unaffected by storage at room temperature with light exposure for up to 8 or 24 h, respectively, but bilirubin levels decreased when specimens were stored at room temperature with light exposure for 24 or 48 h, respectively (both p<0.01). However, these decreases were within the allowable error (20%) of the assay.

   Biospecimens

   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Small molecule	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	<2 h 4 h 8 h 24 h 48 h
   Storage	Storage temperature	3 degrees C 22 degrees C
   Storage	Storage conditions	Light protected Exposed to artificial light
   Preaquisition	Biomarker level	Normal bilirubin levels Elevated bilirubin levels

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