Studies on the effects of heparin products on pregnancy-associated plasma protein A.

Author(s): Wittfooth S, Tertti R, Lepäntalo M, Porela P, Qin QP, Tynjälä J, Inkinen O, Perttilä J, Airaksinen KE, Pettersson K

Publication: Clin Chim Acta, 2011, Vol. 412, Page 376-81

PubMed ID: 21094153 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of intravenous and subcutaneous administration of anticoagulants on PAPP-A levels in serum specimens. All specimens were stored at -70 degrees C until analysis.

   Summary of Findings:

   Intravenous administration of LMWH or UFH resulted in a rapid increase in free PAPP-A levels in serum specimens taken 5 or 10 minutes after administration of the anticoagulant, 85% of which was then cleared with a half-life of 13.1 minutes. Subcutaneous administration of LMWH also resulted in a smaller and slower increase in free PAPP-A levels in serum than that observed with intravenous anticoagulant administration. Intravenous bivalirudin and gender had no effects on free PAPP-A. Complexed PAPP-A levels were unaffected by anticoagulants. In acute coronary syndrome patients, the molecular size of free PAPP-A was larger in heparin plasma than in serum. Free PAPP-A extracted from a plaque tissue specimen with LMWH was also found to have a larger molecular size than PAPP-A extracted with sodium chloride. Further, recombinant PAPP-A diluted in serum with LMWH or in heparin plasma had a larger molecular size than recombinant PAPP-A in serum with no LMWH.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma
   • Tissue - Blood Vessel
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Cardiovascular Disease
   • Coronary Artery Disease
   • Other diagnoses
   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Immunoassay
   Protein	Size-exclusion chromatography

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Time of biospecimen collection	1 h prior to administration Time 0 5 min post administration 10 min post administration 15 min post administration 30 min post administration 40 min post administration 60 min post administration 70 min post administration 2 h post administration 4 h post administration 6 h post administration 12 h post administration
   Preaquisition	Other drugs	Intravenous low molecular weight heparin Intravenous unfractionated heparin Subcutaneous low molecular weight heparin Intravenous bivalirudin
   Preaquisition	Diagnosis/ patient condition	Haemodialysis patient Abdominal aortic aneurysm Atherosclerosis Infection Vascular surgery Multiple trauma Cerebrovascular attack Acute coronary syndrome
   Preaquisition	Patient gender	Female Male
   Biospecimen Acquisition	Biospecimen location	Serum Plasma Plaque tissue
   Biospecimen Aliquots and Components	Blood and blood products	Serum Plasma

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