Proguanylin and prouroguanylin--assay evaluation and clinical analyte characterization.
Author(s): Kaar G, Dieplinger B, Gabriel C, Haltmayer M, Mueller T
Publication: Clin Chim Acta, 2011, Vol. 412, Page 2277-83
PubMed ID: 21888901 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
-
Study Purpose
The purpose of this study was to determine the effects of storage duration at -80 degrees C on the levels of proguanylin and prouroguanylin in plasma. EDTA-plasma was diluted 5-fold prior to measurement. Inter- and intra-individual variability were determined among specimens that were collected weekly, from 22 healthy individuals, in a seated position, between 8 and 10 AM. Gender and age differences were determined using specimens from 125 blood donors.
Summary of Findings:
Storage for 2 months at -80 degrees C did not affect proguanylin or prouroguanylin concentrations in EDTA-plasma. The intraindividual coefficients of variance (CV) were 9% for proguanylin and 21% for prouroguanylin, and the interindividual variabilities were 30% for proguanylin and 26% for prouroguanylin. Males had higher levels of proguanylin than females (7.4 ng/mL versus 6.8 ng/mL p=0.011), but gender had no effect on levels of prouroguanylin. In addition, patient age had no effects on levels of proguanylin or prouroguanylin.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
- Normal
Platform:
Analyte Technology Platform Protein ELISA Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 days
1-20 days
2 months
Biospecimen Acquisition Time of biospecimen collection Week 1
Week 2
Week 3
Week 4
Week 5
Week 6
Biospecimen Aliquots and Components Biospecimen components 10.5 ng/mL proguanylin
24.9 ng/mL proguanylin
7.0 ng/mL prouroguanylin
13.6 ng/mL prouroguanylin
Preaquisition Patient gender Female
Male
Preaquisition Patient age 18-65 years