Interference of deferasirox with assays for serum iron and serum unsaturated iron binding capacity during iron chelating therapy.

Author(s): Ikuta K, Ito S, Tanaka H, Sasaki K, Torimoto Y, Fujiya M, Kohgo Y

Publication: Clin Chim Acta, 2011, Vol. 412, Page 2261-6

PubMed ID: 21884685 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of the addition of the oral iron chelator DFX on measured sFe and UIBC using 4 different assay systems with the 7180 clinical analyzer. Serum was obtained from 4 different institutions. No details of handling or preservation were provided.

   Summary of Findings:

   Adding up to 300 uM of iron-free DFX to pooled serum had no effects on the measurement of sFe using any of the 4 analyzer reagents. Adding iron-free DFX to pooled serum resulted in a concentration-dependent increase in UIBC for all analyzer reagents tested, but the increase was minimal (approximately 1%) using Ferene.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Electrolyte/Metal	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Clinical chemistry/auto analyzer Specific	Reaction solution	FerroZine Ferene Nitroso-PSAP Bathophenanthroline
   Biospecimen Aliquots and Components	Biospecimen components	0 uM DFX 60 uM DFX 120 uM DFX 180 uM DFX 240 uM DFX 300 uM DFX

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