Pre-analytical and analytical considerations for the determination of plasma renin activity.

Author(s): Brossaud J, Corcuff JB

Publication: Clin Chim Acta, 2009, Vol. 410, Page 90-2

PubMed ID: 19766575 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of blood storage temperature and assay duration on PRA as determined by changes in angiotensin I concentrations. The blank used in the PRA assay contained the concentration of angotensin I in specimens assayed at 4 at the relevant reaction time instead of 37 degrees C. All specimens were frozen before analysis.

   Summary of Findings:

   When blood from 14 subjects was stored for 5 h at 25 degrees C, PRA was significantly lower than levels measured in specimens stored for 5 h at 4 degrees C (p<0.002). However, this decrease could be attributed to a more than 50% increase in the activity of the blank (4 degree C reaction condition) and not a change in the angiotensin I concentration (37 degree C reaction condition). The sensitivity and the precision of the PRA assay increased when the incubation time was increased from 1.5 to 18 h.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Enzyme assay
   Protein	Radioimmunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	4 degrees C 25 degrees C
   Enzyme assay Specific	Incubation time	1.5 h 18 h

   View more