Effect of sample collection on alpha-galactosidase A enzyme activity measurements in dried blood spots on filter paper.

Author(s): Olivova P, van der Veen K, Cullen E, Rose M, Zhang XK, Sims KB, Keutzer J, Browning MF

Publication: Clin Chim Acta, 2009, Vol. 403, Page 159-62

PubMed ID: 19245803 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this paper was to determine the effects of sample collection method on AGAL activity in dried blood spots from normal individuals and patients with Fabry disease. The effects of storage and anticoagulant concentration on AGAL in tube-collected specimens were also examined.

   Summary of Findings:

   No difference in AGAL activity was found with direct spotting of blood collected by venipuncture or lancet. However, AGAL activity was up to 68% higher in specimens collected in EDTA tubes and spotted 24-72 h later (p=0.0007). Storage duration and EDTA concentration did not significantly effect AGAL activity in tube-collected blood specimens, but the coefficient of variance (CV) was higher in specimens stored for 72 h prior to spotting than in those stored for 24 h. In Fabry disease patients, AGAL activity was significantly lower. No significant effects of gender or age were observed, but some females had levels that overlapped the normal range. In conclusion, AGAL activity is dependent on collection method, and in blood specimens collected in tubes, anticoagulant concentration and storage for up to 72 h do not influence activity.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Protein	Enzyme assay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	EDTA vacutainer tube Filter paper
   Biospecimen Acquisition	Method of fluid acquisition	Finger/heel prick sampling Venipuncture
   Storage	Storage temperature	4 degrees C
   Storage	Storage duration	24 h 48 h 72 h
   Preaquisition	Patient gender	Female Male
   Preaquisition	Diagnosis/ patient condition	Fabry disease Normal
   Biospecimen Aliquots and Components	Aliquot size/volume	2 mL 5 mL 10 mL
   Biospecimen Acquisition	Anatomical location of blood draw	Capillary Vein

   View more