Effect of anticoagulants on plasma homocysteine determination.

Author(s): Caliskan S, Kuralay F, Onvural B

Publication: Clin Chim Acta, 2001, Vol. 309, Page 53-6

PubMed ID: 11408006 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of anticoagulant and storage temperature prior to centrifugation on Hcy levels in plasma. After experimental storage, all specimens were frozen at -70 degrees C for 15 days prior to analysis. To achieve a 0 degrees C storage temperature, specimens were kept on crushed ice.

   Summary of Findings:

   Significantly higher Hcy levels were measured in specimens collected with K3EDTA than with sodium citrate (p=0.02) or sodium fluoride and potassium oxalate (p=0.000), and the latter two specimen types were not significantly different from one another. Hcy levels decreased compared to baseline (measured after immediate centrifugation) when sodium fluoride/potassium oxalate specimens were kept on ice for 3 h prior to centrifugation (p=0.043), but the same was not observed for K3EDTA or sodium citrate specimens stored on ice. Hcy levels increased when specimens with any of the three anticoagulants were held at room temperature for 3 hours prior to centrifugation compared to baseline (all p=0.000).

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	0 degrees C 22 degrees C
   Storage	Storage duration	0 h 3 h
   Biospecimen Acquisition	Anticoagulant	Potassium EDTA Sodium citrate Sodium fluoride/potassium oxalate
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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