Stability of urine creatinine.
Author(s): Spierto FW, Hannon WH, Gunter EW, Smith SJ
Publication: Clin Chim Acta, 1997, Vol. 264, Page 227-32
PubMed ID: 9293380 PubMed Review Paper? No
Purpose of Paper
This paper investigated the effects of storage duration and temperature on creatinine levels in thawed urine specimens.
Conclusion of Paper
Urine creatinine concentrations were unaffected by storage for 30 days at 4˚C, 8 days at 25˚C, or 2 days at 55˚C. Creatinine levels were 15% lower after 10 days of storage at 37˚C compared to aliquots analyzed immediately after thawing.
Studies
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Study Purpose
This study investigated the effects of storage duration and temperature on creatinine levels in thawed urine specimens. Urine from ten healthy adults was immediately placed on ice and aliquoted. Aliquots were frozen at -70˚C. To test the effects of storage, urine aliquots were thawed and tested immediately or stored at 4˚C, 25˚C, 37˚C, or 55˚C for 1 h, 20 h, 2 days, 5 days, 10 days, 22 days, and 30 days before being refrozen at -70˚C. Creatinine levels were measured using a two-point enzymatic creatinine assay on a clinical chemistry analyzer.
Summary of Findings:
Urine creatinine concentrations were unaffected by storage for 30 days at 4˚C, 8 days at 25˚C, or 2 days at 55˚C. Creatinine levels were 15% lower after 10 days at 37˚C compared to aliquots analyzed immediately after thawing.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Small molecule Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage temperature 4˚C
25˚C
37˚C
55˚C
Storage Freeze/thaw cycling 0 cycles
1 cycle
Storage Storage duration 1 h
20 h
2 days
5 days
10 days
22 days
30 days