Storage and stability of folate and vitamin B-12 in plasma and blood samples.
Author(s): Kubasik NP, Graham M, Sine HE
Publication: Clin Chim Acta, 1979, Vol. 95, Page 147-9
PubMed ID: 509725 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to evaluate the effects of storage temperature and duration on folate and vitamin B 12 levels in heparin-plasma and erythrocytes.
Conclusion of Paper
Erythrocyte levels of folate were relatively stable at -6 degrees C for 2 weeks, but degraded rapidly with storage at 4 or 22 degrees C. Plasma folate was also stable when stored at -6 degrees C and unstable at 22 degrees C, where a high degree of variability was observed between 7 and 14 days. At 4 degrees C, a slight decrease in plasma folate levels was observed after one day, but during subsequent storage, levels remained relatively stable. Vitamin B12 levels in plasma were not affected by storage at any temperature.
Studies
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Study Purpose
The purpose of this study was to evaluate the effects of storage temperature and duration on folate and vitamin B 12 levels in heparin plasma and erythrocytes. Erythrocytes were obtained by lysing whole blood with ascorbic acid followed by immediate centrifugation.
Summary of Findings:
Erythrocyte levels of folate were relatively stable at -6 degrees C for 2 weeks, but degraded rapidly with storage at 4 or 22 degrees C. Plasma folate was also stable when stored at -6 degrees C and unstable at 22 degrees C, where a high degrees of variability was observed between 7 and 14 days. At 4 degrees C, a slight decrease in plasma folate levels was observed after one day, but during subsequent storage, levels remained relatively stable. Vitamin B12 levels in plasma were not affected by storage at any temperature.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Small molecule Radioassay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Blood and blood products Plasma
Red blood cells
Storage Storage duration 1 day
7 days
14 days
Storage Storage temperature -6 degrees C
4 degrees C
22 degrees C