The effect of blood anticoagulants on serum and plasma amylase activities.

Author(s): MCGEACHIN RL, DAUGHERTY HK, HARGAN LA, POTTER BA

Publication: Clin Chim Acta, 1957, Vol. 2, Page 75-7

PubMed ID: 13447218 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of anticoagulant on amylase activity in serum and plasma specimens. Blood specimens were drawn into tubes containing anticoagulants, and plasma was separated and stored at 2 degrees C until amylase was assayed.

   Summary of Findings:

   14% and 17% less amylase activity was detected in potassium oxalate or sodium citrate anticoagulated plasma, respectively, than was found in serum. However, heparin-plasma had similar levels of amylase activity to serum. The authors report that even when potassium oxalate or sodium citrate was added to serum just prior to analysis, amylase activity was decreased.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Colorimetric assay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Biospecimen Acquisition	Anticoagulant	Heparin Sodium citrate None Potassium oxalate

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