Preanalytical storage does not affect 99th percentile cardiac troponin T concentrations measured with a high-sensitivity assay.
Author(s): Gillis JM, Dunselman P, Jarausch J, de Jong N, Cobbaert CM
Publication: Clin Chem, 2013, Vol. 59, Page 442-3
PubMed ID: 23220225 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of delayed blood processing and storage of plasma and serum on levels of cTnT1 in blood from patients with suspected myocardial damage. Blood was collected into Venosafe serum tubes or lithium heparin gel tubes. Blood was processed immediately or after storage at room temperature for 3 or 5 h, and the plasma and serum were frozen. Additional plasma and serum specimens were stored at room temperature for 1, 2, 6 or 24 h, 2-8 degrees C for 1, 2, 6 or 24 h, or -20 degrees C for 24 h, 1 week, 4 weeks, or 3 months, after which all specimens were frozen at -80 degrees C for 3 months.
Summary of Findings:
cTnT1 levels in serum or lithium heparin plasma were not affected by storage of whole blood for up to 5 h before processing (longest duration examined). Further, there was no effect of storage of plasma or serum at room temperature, 2-8 degrees C, or -20 degrees C on cTnT1 levels. cTnT1 levels were comparable in lithium heparin plasma and serum.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Cardiovascular Disease
- Not specified
Platform:
Analyte Technology Platform Peptide Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 h
1 h
2 h
3 h
5 h
6 h
24 h
1 week
4 weeks
3 months
Storage Storage temperature Room temperature
2-8 degrees C
-20 degrees C
-80 degrees C
Biospecimen Aliquots and Components Blood and blood products Plasma
Serum
Whole blood
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated