The effects of sampling procedures and storage conditions on short-term stability of blood-based biochemical markers of bone metabolism.

Author(s): Stokes FJ, Ivanov P, Bailey LM, Fraser WD

Publication: Clin Chem, 2011, Vol. 57, Page 138-40

PubMed ID: 20974796 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of anticoagulant type, delayed centrifugation, and storage of plasma and serum at room temperature or 2-8 degrees C on the measurement of Beta-CTX, P1NP, OC, bone ALP, and PTH in specimens from patients with metabolic bone disease. All serum and plasma specimens were frozen at -70 degrees C until analysis.

   Summary of Findings:

   Baseline values of the analytes were highly correlated and not statistically different between lithium heparin plasma, EDTA plasma, or serum. P1NP and bone ALP were stable in serum and plasma, regardless of anticoagulant, after 48 h room temperature delays in centrifugation of whole blood, storage of serum or plasma for 7 days at room temperature, or storage for 28 days at 2-8 degrees C (the longest durations tested). PTH concentrations were stable in lithium heparin and EDTA plasma during all storage conditions, however levels decreased in serum when whole blood was held at room temperature for 24 h prior to centrifugation, when serum was held at room temperature for 48 h, or when serum was held at 2-8 degrees C for 14 days. OC and Beta-CTX showed varying stability based on storage temperature, duration, anticoagulant type and use of plasma versus serum, but they were each generally more stable in EDTA plasma than lithium heparin plasma or serum.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Peptide	Clinical chemistry/auto analyzer
   Protein	Clinical chemistry/auto analyzer
   Protein	Immunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Diagnosis/ patient condition	Metabolic bone disease
   Storage	Time at room temperature	1 h 2 h 4 h 8 h 24 h 48 h
   Storage	Storage duration	2 h 4 h 8 h 24 h 48 h 7 d 14 d 28 d
   Storage	Storage temperature	Room temperature 2-8 degrees C
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Biospecimen Acquisition	Anticoagulant	EDTA Lithium heparin
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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