Short- and long-term cardiac troponin I analyte stability in plasma and serum from healthy volunteers by use of an ultrasensitive, single-molecule counting assay.
Author(s): Wu AH, Shea E, Lu QT, Minyard J, Bui K, Hsu JC, Agee SJ, Todd J
Publication: Clin Chem, 2009, Vol. 55, Page 2057-9
PubMed ID: 19729470 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of delayed centrifugation of whole blood and room temperature storage of serum on the measurement of cTnI using the ultrasensitive Erenna cTnI immunoassay in EDTA-plasma and serum from healthy individuals and patients with chest pain without elevated cTnI. All specimens were frozen at -80 degrees C after room temperature storage. Effects of storage on serum and plasma levels were not differentiated by the authors.
Summary of Findings:
In healthy subjects, storing specimens at room temperature for 6 and 48 h led to 11% and 16% coefficients of variance (CV), respectively. Further, the CV of cTn1 in over 1 h in healthy subjects and 6 h in chest pain patients were <1% and <3%, respectively. Over 48 h storage a 0.015-0.339 ng/ul per h increase in cTnI levels were observed, but the authors report that these changes would not be clinically relevant. The authors report that stability was similar in whole blood, EDTA-plasma and serum.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
- Not specified
- Cardiovascular Disease
Platform:
Analyte Technology Platform Protein Immunoassay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Time at room temperature 0 h
1 h
2 h
4 h
6 h
24 h
48 h
Biospecimen Aliquots and Components Blood and blood products Plasma
Serum
Whole blood
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated