Preanalytical stability of 25(OH)-vitamin D3 in human blood or serum at room temperature: solid as a rock.
Author(s): Wielders JP, Wijnberg FA
Publication: Clin Chem, 2009, Vol. 55, Page 1584-5
PubMed ID: 19541868 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of storage conditions on the stability of 25(OH)-vitamin D3 in serum. After exposure to different storage conditions, all serum specimens were stored at -20 degrees C until analysis.
Summary of Findings:
Light and up to 4 freeze-thaw cycles did not significantly impact the stability of 25(OH)-vitamin D3. Storage of serum at -20 degrees C for 2 months resulted in a 4.0% decrease in 25(OH)-vitamin D3 concentration, and storage of serum at 6 degrees C for 7 days resulted in an average decrease in 25(OH)-vitamin D3 concentration of only 1.8%. While storage of serum at room temperature resulted in a 4.5% decrease in 25(OH)-vitamin D3 concentration, storage of whole blood at room temperature for 3 days resulted in only a 2.3% decrease. The authors conclude that whole blood can be stored at room temperature for up to 3 days without significantly affecting 25(OH)-vitamin D3 stability, but that serum is best stored frozen or in the refrigerator.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Small molecule Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage conditions Light
Dark
Storage Storage temperature 6 degrees C
Room temperature
-20 degrees C
Biospecimen Aliquots and Components Blood and blood products Serum
Whole blood
Storage Freeze/thaw cycling 0 cycles
4 cycles
Storage Storage duration 0 h
7 h
1 d
2 d
3 d
5 d
7 d
2 months
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated