Preanalytical factors affecting RIA measurement of plasma kisspeptin.

Author(s): Ramachandran R, Patterson M, Murphy KG, Dhillo WS, Patel S, Kazarian A, Ghatei MA, Bloom SR

Publication: Clin Chem, 2008, Vol. 54, Page 615-7

PubMed ID: 18310154 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study examined the stability of kisspeptin immunoreactivity (IR) in serum specimens subjected to a preprocessing room temperature delay of 0-4 h. Kisspeptin IR levels in serum and immediately processed plasma specimens were also compared. Biospecimens were collected from healthy pregnant women for all studies.

   Summary of Findings:

   In serum specimens, detectable kisspeptin IR was limited to samples processed immediately (0 h). Kisspeptin IR levels were significantly lower in immediately processed serum specimens compared to corresponding plasma specimens.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Pregnant

   Platform:

   Analyte	Technology Platform
   Peptide	Radioimmunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	0 h 1 h 2 h 4 h
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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2. Study Purpose

   The purpose of this study was to investigate whether a preprocessing room temperature delay and the type of anticoagulant used impacts plasma kisspeptin immunoreactivity (IR), as determined by radioimmunoassay. Biospecimens were collected from healthy pregnant women.

   Summary of Findings:

   Although not significant, plasma kisspeptin IR was lower in specimens treated with citrate compared to EDTA or lithium heparin. IR was inversely proportional to the duration of a pre-processing delay at room temperature, with a significant decrease (compared to 0 h controls) after 1 or 2 h in SST or citrate and lithium heparin treated specimens, respectively. Plasma specimens treated with EDTA were stable at room temperature for up to 4 h.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Pregnant

   Platform:

   Analyte	Technology Platform
   Peptide	Radioimmunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	0 h 1 h 2 h 4 h
   Biospecimen Acquisition	Anticoagulant	EDTA Citrate Lithium heparin None
   Biospecimen Acquisition	Type of collection container/solution	Serum separator (SST) Serum clot activator (S)
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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3. Study Purpose

   The purpose of this study was to determine if multiple freeze-thaw cycles impact kisspeptin immunoreactivity (IR) in EDTA or lithium heparin treated plasma specimens. Biospecimens were collected from healthy pregnant women, and samples were processed immediately.

   Summary of Findings:

   Although data was not shown, the authors report freeze-thaw cycling up to 4 times, did not significantly affect kisspeptin IR in plasma treated with EDTA or lithium heparin.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Pregnant

   Platform:

   Analyte	Technology Platform
   Peptide	Radioimmunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Anticoagulant	EDTA Lithium heparin
   Storage	Freeze/thaw cycling	0 cycles 1 cycle 2 cycles 3 cycles 4 cycles

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