Effect of collection tube type and preanalytical handling on myeloperoxidase concentrations.
Author(s): Shih J, Datwyler SA, Hsu SC, Matias MS, Pacenti DP, Lueders C, Mueller C, Danne O, Möckel M
Publication: Clin Chem, 2008, Vol. 54, Page 1076-9
PubMed ID: 18509013 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
-
Study Purpose
The purpose of this study was to determine the effects of collection tube type and storage duration prior to centrifugation either in an ice bath or at room temperature on MPO concentration in normal blood specimens.
Summary of Findings:
EDTA and citrate plasma tubes gave the lowest concentrations of MPO and were not significantly different. Average MPO concentrations were significantly higher in heparin (10%) and serum specimens (up to 100%) (p<0.001). A spike recovery experiment showed that the higher MPO concentrations were not due to direct anticoagulant interference. MPO concentration was stable when whole blood was kept on ice for 8 hours or at room temperature for 2 hours prior to centrifugation in either EDTA or EDTA plasma preparation tubes. In citrate and lithium heparin tubes, MPO concentrations were only stable when whole blood was kept on ice for 2 hours prior to centrifugation and were not stable when whole blood was stored at room temperature.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Protein Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Blood and blood products Plasma
Serum
Biospecimen Acquisition Type of collection container/solution Serum (glass)
Serum (plastic)
Serum separator
K2 EDTA
K2 EDTA plasma preparation
Lithium heparin (glass)
Lithium heparin (plastic)
Lithium heparin plasma separator
Sodium heparin
Sodium citrate
Storage Storage temperature 0 degrees C
RT
Storage Storage duration 0 h
2 h
8 h
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated
-
Study Purpose
The purpose of this study was to determine the effects of storage of plasma or serum at room temperature or at 2-8 degrees C, and freeze/thaw cycling following centrifugation on MPO concentration in normal specimens.
Summary of Findings:
Storage of plasma or serum at room temperature for 2 days, at 2-8 degrees C for 8 days, or during up to 3 freeze/thaw cycles only altered MPO concentrations by 7% or less from baseline in any tested tube type with EDTA tubes performing the best.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Protein Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Blood and blood products Plasma
Serum
Biospecimen Acquisition Type of collection container/solution Serum separator
K2 EDTA plasma preparation
Lithium heparin plasma separator
Storage Storage temperature 2-8 degrees C
Storage Storage duration 0 d
1 d
2 d
5 d
8 d
Storage Time at room temperature 0 h
3.5 h
24 h
48 h
Storage Freeze/thaw cycling 0 cycles
1 cycle
2 cycles
3 cycles
-
Study Purpose
The purpose of this study was to determine the effects of collection tube type on MPO concentration in specimens collected from patients presenting with chest pain.
Summary of Findings:
EDTA and citrate plasma tubes were similar and gave the lowest concentrations of MPO. Lithium heparin and serum samples had average MPO concentrations of between 2.5 and 3 times those levels found in EDTA and citrate tubes.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Cardiovascular Disease
Platform:
Analyte Technology Platform Protein Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Blood and blood products Plasma
Serum
Biospecimen Acquisition Type of collection container/solution Serum separator
K2 EDTA
Lithium heparin plasma separator
Citrate
Storage Storage temperature 2-8 degrees C