Preanalytic influence of sample handling on SELDI-TOF serum protein profiles.

Author(s): Timms J F, Arslan-Low E, Gentry-Maharaj A, Luo Z, T'Jampens D, Podust V N, Ford J, Fung E T, Gemmerman A, Jacobs A, Menon U

Publication: Clin Chem, 2007, Vol. 53, Page 645

PubMed ID: 17303688 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this paper was to determine if serum protein profiles are adversely affected by different collection tube types, clotting times, transport temperatures and durations, and storage conditions, as measured by SELD-TOF-MS. The method of specimen transportation was not specified.

   Summary of Findings:

   Principal components analysis revealed partial grouping of samples based upon storage temperature (room temperature versus wet ice) prior to centrifugation, while no differences were present among samples transported on ice regardless of duration (3 versus 6 h). Statistical analysis revealed that clotting time also influenced serum protein profiles (5 versus 60 min), while the type of storage container had no effect. Effects due to collection tube type could not be extrapolated. Effects of serum handling variables were protein specific. The authors conclude that storage on wet ice is critical in the preservation of serum protein profiles, although a clotting time of 60 minutes yielded less profile variability.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	SELDI-TOF MS

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	Greiner gel tube Becton Dickinson red-top tube
   Storage	Type of storage container	Cryovial Straw
   Storage	Time at room temperature	5 min clot time 1 h clot time
   Storage	Storage duration	2 h 3 h 6 h 30 h
   SELDI-TOF MS Specific	Technology platform	CM10 array IMAC 30 array H50 array
   Storage	Specimen transport duration/condition	Vertical on ice Room temperature 3 h 6 h 30 h
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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