Potential interferences from blood collection tubes in mass spectrometric analyses of serum polypeptides.

Author(s): Drake SK, Bowen RA, Remaley AT, Hortin GL

Publication: Clin Chem, 2004, Vol. 50, Page 2398-401

PubMed ID: 15563493 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine whether different types of blood collection tubes shed exogenous molecules capable of interfering with MALDI-TOF mass spectrometry peptide analysis. Both serum and plasma collection tubes were assessed by incubating saline in respective tubes at room temperature for 4 hours; biospecimens were not directly investigated.

   Summary of Findings:

   With the exception of no-additive glass tubes all serum collection tubes generated a series of MALDI-TOF mass spectrometry peaks within the m/z range of 1000 to 3000; tubes from two different manufacturers produced similar results. Conversely, the majority of plasma collection tubes investigated did not yield peptide peaks suggesting minimal interference, including EDTA-containing plasma tubes, no-additive glass tubes with a glycerol coated stopper, and tubes containing citrate or heparin additives. Research-use plasma separator tubes containing protein stabilizing additives generated several peaks within the m/z range of 1050-6880.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Peptide	MALDI-TOF MS

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	10 ml Vacutainer Clot Activator Plus Plastic 7 ml Vacutainer SST Gel and Clot Activator 3.5 ml Vacutainer SST Gel and Clot Activator 8.5 ml Plus Plastic EDTA separator 2.5 ml Vacuette Z serum separator (clot activator) 4 ml Vacuette Z serum separator (clot activator) 8 ml Vacuette Z serum separator (clot activator) 10 x 65 mm Glass Vacutainer 6 ml Vacutainer Plus sodium heparin 4.5 ml Vacutainer buffered sodium citrate 3 ml Vacutainer Plus 5.4 mg K2EDTA

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