Immunoluminometric assay for the midregion of pro-atrial natriuretic peptide in human plasma.
Author(s): Morgenthaler NG, Struck J, Thomas B, Bergmann A
Publication: Clin Chem, 2004, Vol. 50, Page 234-6
PubMed ID: 14709661 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to determine the effects of patient age and gender, anticoagulant, storage, and freeze-thaw cycling on the levels of the prohormone of atrial natriuretic peptide (proANP) in plasma.
Conclusion of Paper
ProANP levels in EDTA-plasma were stable for at least 48 h at 4 degrees C, 6 months at -20 degrees C, and through four freeze-thaw cycles. There were no differences between male and female proANP levels when all ages were considered together, but in the 26-35 year age group, proANP levels were higher in females than males. ProANP levels were significantly correlated with age and were significantly higher in the oldest age group. While the authors report no difference between citrated and EDTA-plasma, they report nonsystematic increases of 300% in some sera specimens and in heparinated plasma.
Studies
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Study Purpose
The purpose of this study was to determine the effects of patient age and gender, anticoagulant, storage, and freeze-thaw cycling on the levels of proANP in plasma.
Summary of Findings:
Up to 48 h of storage at 4 degrees C had no effect on proANP levels in 8 EDTA-plasma specimen, but after 14 days, 3 of 5 specimens showed no difference in proANP levels while the other 2 had a more than 20% increase in proANP levels. ProANP levels were unaffected by 6 months of storage at -20 degrees C or four freeze-thaw cycles. While the authors report no difference between citrated and EDTA-plasma, they report nonsystematic increases of 300% in some sera specimens and in heparinated plasma. There were no differences between male and female proANP levels when all ages were considered together, but in the 26-35 year age group, proANP levels were higher in females than males (p=0.02). ProANP levels were significantly correlated with age (r=0.29; p<0.001), and were significantly higher in the oldest age group (p<0.001).
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Peptide Immunoassay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Patient age 18-25 years
26-35 years
36-45 years
46-55 years
56-65 years
Preaquisition Patient gender Female
Male
Storage Storage temperature -20 degrees C
4 degrees C
Storage Storage duration 0 days
2 days
14 days
6 months
Storage Freeze/thaw cycling 0 cycles
4 cycles
Biospecimen Acquisition Anticoagulant Citrate
EDTA
Heparin
None
Biospecimen Aliquots and Components Blood and blood products Serum
Plasma