Effects of blood-processing protocols on cell-free DNA quantification in plasma.

Author(s): Swinkels DW, Wiegerinck E, Steegers EA, de Kok JB

Publication: Clin Chem, 2003, Vol. 49, Page 525-6

PubMed ID: 12600978 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of blood volume and a second centrifugation step in the preparation of plasma on the concentration of albumin DNA in plasma from pregnant and non-pregnant women. Blood was processed within 3 h, and the resultant plasma was stored at -20 degrees C for at least 1 month before DNA extraction.

   Summary of Findings:

   The concentration of albumin DNA in plasma obtained from 7 mL EDTA tubes centrifuged at 800 x g for 10 min was comparable to that in plasma obtained after an additional centrifugation at 16000 x g for 5 min. When the blood volume was increased to 10 mL, the albumin concentration was higher in plasma obtained by a single centrifugation at 800 x g than in plasma obtained after centrifugation at 800 x g and 16000 x g, regardless of whether the second centrifugation was before or after storage at -20 degrees C for more than a month. Further, after a second centrifugation step, plasma from 10 mL tubes contained comparable concentrations of albumin DNA to plasma from 7 mL tubes.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Plasma

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal
   • Pregnant

   Platform:

   Analyte	Technology Platform
   DNA	Real-time qPCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Real-time qPCR Specific	Targeted nucleic acid	Albumin
   Biospecimen Aliquots and Components	Aliquot size/volume	7 mL 10 mL
   Biospecimen Aliquots and Components	Centrifugation	Different number of centrifugation steps compared

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