Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Effect of anticoagulants and cell separation media as preanalytical determinants on zymographic analysis of plasma matrix metalloproteinases.

Author(s): Mannello F, Luchetti F, Canonico B, Papa S

Publication: Clin Chem, 2003, Vol. 49, Page 1956-7

PubMed ID: 14578336 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to assess the potential influence anticoagulants and cell separation media have on matrix metalloproteinase (MMP) levels in plasma and leukocyte specimens.

   Summary of Findings:

   Circulating MMP expression was influenced both by the type and concentration of anticoagulant used. MMP expression was lower in K2EDTA compared to lithium heparin plasma specimens, and K2EDTA resulted in a dose-dependent decrease and increase in MMP-9 and MMP-2 levels, respectively. Analysis of plasma obtained from a single volunteer served to reduce variability and highlighted increased levels of proMMP-2 and proMMP-9 in buffered/acidic citrate plasma or K2EDTA and sodium fluoride/potassium oxalate plasma specimens, respectively.

   Biospecimens

   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Zymography

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Anticoagulant	Buffered acidic citrate Citrate-phosphate-dextrose Lithium heparin Potassium EDTA Sodium fluoride/potassium oxalate None Acid-citrate-dextrose

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