Changes in concentration of DNA in serum and plasma during storage of blood samples.

Author(s): Jung M, Klotzek S, Lewandowski M, Fleischhacker M, Jung K

Publication: Clin Chem, 2003, Vol. 49, Page 1028-9

PubMed ID: 12766024 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of collection tube type and storage of whole blood prior to centrifugation on levels of beta-globin DNA in plasma and serum from healthy blood donors. Plasma and serum were frozen at -80 degrees C until DNA extraction. DNA was extracted with NucleoSpin Blood DNA kit and stored at -20 degrees C until analysis.

   Summary of Findings:

   Serum had 2.3-fold higher beta-globin concentrations than plasma (p<0.05), but the use of serum tubes containing kaolin-coated plastic granulate coagulation accelerator rather than plain serum tubes had no effect on beta-globin concentration. While pre-centrifugation storage of whole blood had no effect on beta-globin concentration in plasma, storage of whole blood for 2 h at room temperature prior to centrifugation led to significantly higher beta-globin concentrations in serum than when blood was centrifuged immediately (p<0.05). Further, a non-significant trend toward higher concentrations of beta-globin in serum was observed when whole blood was stored at 4 degrees C for 24 h prior to centrifugation compared to when specimens were centrifuged immediately.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   DNA	Real-time qPCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 h 2 h 4 h 8 h 24 h
   Storage	Storage temperature	4 degrees C Room temperature
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Biospecimen Acquisition	Type of collection container/solution	Potassium EDTA Monovette Coagulation accelerator serum tube Plain serum monovette
   Real-time qPCR Specific	Targeted nucleic acid	Beta-globin
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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