Does centrifugation cause the ex vivo release of DNA from blood cells?
Author(s): Lui YY, Chik KW, Lo YM
Publication: Clin Chem, 2002, Vol. 48, Page 2074-6
PubMed ID: 12407004 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effect of centrifugation speed on the cell-free DNA concentration in plasma.
Conclusion of Paper
The amount of DNA obtained from plasma was not affected by the centrifugation speed used for plasma separation among specimens from healthy individuals and bone marrow transplant patients.
Studies
-
Study Purpose
The purpose of this study was to determine the effect of centrifugation speed on the cell-free DNA concentration in plasma. Peripheral blood was collected from 6 bone marrow transplant and 10 healthy patients into EDTA tubes and centrifuged for 10 min at 400, 800, 1500, 3000, or 16000 x g. Plasma was then filtered to remove cells. DNA was extracted from plasma using the QIAamp blood kit. DNA was quantified by amplification of the beta-globin gene.
Summary of Findings:
The amount of DNA obtained from plasma was not affected by the centrifugation speed used for plasma separation among specimens from healthy individuals and bone marrow transplant patients.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Other diagnoses
- Normal
Platform:
Analyte Technology Platform DNA Real-time qPCR Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Centrifugation Multiple speeds compared
Preaquisition Diagnosis/ patient condition Healthy
Bone marrow transplant patient
Real-time qPCR Specific Targeted nucleic acid Beta-globin