Measurement of circulating forms of prostate-specific antigen in whole blood immediately after venipuncture: implications for point-of-care testing.

Author(s): Piironen T, Nurmi M, Irjala K, Heinonen O, Lilja H, Lövgren T, Pettersson K

Publication: Clin Chem, 2001, Vol. 47, Page 703-11

PubMed ID: 11274021 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of delayed analysis, exercise prior to specimen collection, and the use of EDTA or heparin anticoagulated whole blood or plasma versus serum to measure PSA-T, PSA-F, and PSA-ACT. The effects of delayed analysis were observed by either measuring the recovery of purified PSA, from spiked blood and serum from females, at defined time points during storage at room temperature, or by measuring endogenous PSA at defined time points after venipuncture. Exercise consisted of 15 minutes on a stationary bike.

   Summary of Findings:

   Riding a stationary bike for 15 minutes prior to specimen collection did not affect the amount of PSA-T, PSA-F, or PSA-ACT measured in EDTA whole blood compared with levels measured in specimens collected before the exercise, even when analysis of the blood was delayed for up to 100 minutes. When purified PSA was used to spike serum from a female, the majority of enzymatic reactivity was gone after a 15 minute delay before analysis. The authors state that the elimination curves of purified PSA-F were similar between serum, EDTA whole blood, heparin whole blood, EDTA plasma, and heparin plasma. For both healthy males and those with prostate cancer or benign prostatic hyperplasia, room temperature storage of heparin whole blood, EDTA whole blood, or serum prior to analysis, for up to 48 h (healthy) or 4 h (patients), did not affect PSA-T, PSA-F, or PSA-ACT (measured for patients only). Very strong correlations in PSA-T and PSA-F values were observed between whole blood and serum specimens.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal
   • Neoplastic - Benign
   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Glycoprotein	Immunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Time of biospecimen collection	Prior to exercise After exercise
   Storage	Time at room temperature	1 min 15 min 30 min 60 min 100 min 2 h 4 h 12 h 48 h
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum Whole blood
   Biospecimen Acquisition	Anticoagulant	EDTA Heparin

   View more