OVX1 radioimmunoassay results are dependent on the method of sample collection and storage.

Author(s): Hogdall EV, Hogdall CK, Kjaer SK, Xu F, Yu Y, Bast RC, Blaakaer J, Jacobs IJ

Publication: Clin Chem, 1999, Vol. 45, Page 692-4

PubMed ID: 10222361 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of delayed centrifugation, storage temperature, and freeze-thaw cycling on the stability of OVX1 in plasma and serum. All specimens were frozen at -20 degrees C until analysis.

   Summary of Findings:

   Compared to serum OVX1 levels in immediately centrifuged specimens (4.0 kilounits/L), significant increases were observed after blood specimens were left at room temperature for 3 h (5.5 kilounits/L) and 48 h (15.0 kilounits/L) prior to centrifugation. However, storage of blood specimens at 4 degrees C for up to 72 hours prior to centrifugation had no effect on serum OVX1 concentrations. Plasma from EDTA blood or heparinated blood showed significantly lower OVX1 concentrations than serum specimens collected in tubes without anticoagulant. Up to 8 freeze-thaw cycles produced clinically insignificant decreases in serum OVX1 concentrations.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Glycoprotein	Radioimmunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	Room temperature 4 degrees C
   Storage	Storage duration	0 h 1 h 2 h 3 h 5 h 7 h 10 h 48 h 72 h
   Biospecimen Acquisition	Anticoagulant	EDTA Heparin None
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Storage	Freeze/thaw cycling	1 cycle 2 cycles 3 cycles 4 cycles 5 cycles 6 cycles 7 cycles 8 cycles
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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