Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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False increases of troponin I attributable to incomplete separation of serum.

Author(s): Nosanchuk JS, Combs B, Abbott G

Publication: Clin Chem, 1999, Vol. 45, Page 714

PubMed ID: 10222366 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of recentrifugation and collection of plasma instead of serum on troponin I levels using the AxSYM assay.

   Summary of Findings:

   Initial levels of Troponin I were higher in serum than in plasma, however, after serum was centrifuged a second time, the troponin I levels were the same as in plasma. The authors believe the antibody used in the AxSYM assay may interact with fibrin resulting in falsely high Troponin I levels when serum separation is incomplete.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Biospecimen Aliquots and Components	Centrifugation	Different number of centrifugation steps compared

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