Effect of serum volume and time of exposure to gel barrier tubes on results for progesterone by Roche Diagnostics Elecsys 2010.

Author(s): Ferry JD, Collins S, Sykes E

Publication: Clin Chem, 1999, Vol. 45, Page 1574-5

PubMed ID: 10471667 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine if the levels of quantifiable hormones (progesterone, estradiol, luteinizing hormone, follicle-stimulating hormone) and proteins of interest (creatine kinase MB isoenzyme, troponin T) fluctuate as a result of serum contact with SST separator gel. Specimens were centrifuged before experimental storage.

   Summary of Findings:

   Progesterone was significantly higher in sera collected and stored in SST Vacutainer tubes containing separator gel compared to plain glass tubes. The observed increase in progesterone was time-dependent, increasing as soon as 2 h post-collection. Serum levels did not differ among tube types for the remainder of the hormone and proteins investigated.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Steroid	Clinical chemistry/auto analyzer
   Protein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	0 h 2 h 4 h 6 h 8 h
   Storage	Type of storage container	SST Vacutainer tube Plain glass Vacutainer tube

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2. Study Purpose

   The purpose of this study was to determine if the artificial increase in quantifiable progesterone levels observed in serum collected in SST Vacutainer tubes is influenced by specimen volume.

   Summary of Findings:

   The increase in quantified progesterone during post-centrifugation storage in the original collection tube was more robust in small volumes of serum (2 and 5 ml) versus larger volumes (10 ml).

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Steroid	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Aliquot size/volume	2 ml 5 ml 10 ml
   Storage	Time at room temperature	0 h 2 h 4 h
   Storage	Type of storage container	SST Vacutainer tubes Plain glass Vacutainer tubes

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