Effect of serum-clot contact time on clinical chemistry laboratory results.
Author(s): Zhang DJ, Elswick RK, Miller WG, Bailey JL
Publication: Clin Chem, 1998, Vol. 44, Page 1325-33
PubMed ID: 9625060 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of delayed separation from the clot on the levels of 63 clinical chemistry analytes in serum. Specimens from at least 4 individuals were used for each analyte. After centrifugation, serum was refrigerated at 4 degrees C until analysis and protected from light when necessary.
Summary of Findings:
Storing serum on the clot for 3 hours at 32 degrees C resulted in decreased levels of glucose that were clinically significant, while levels of albumin and inorganic phosphorus did not show clinically significant increases until serum was stored for 6 h on the clot. Levels of bicarbonate, chloride, C-peptide, iron, HDL, LDL, and total protein remained within clinically acceptable levels after storage of the serum in contact with the clot for 6 h, but between 6-24 h, iron and total protein increased and bicarbonate, chloride, C-peptide, HDL and LDL decreased. Levels of 50 other clinical chemistry analytes were not clinically affected by storage of serum in contact with the clot at 32 degrees C for 24 hours.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Carbohydrate Clinical chemistry/auto analyzer Electrolyte/Metal Clinical chemistry/auto analyzer Glycoprotein Clinical chemistry/auto analyzer Lipid Clinical chemistry/auto analyzer Lipoprotein Clinical chemistry/auto analyzer Peptide Clinical chemistry/auto analyzer Protein Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Steroid Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration <30 min
3 h
6 h
12 h
24 h
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated
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Study Purpose
The purpose of this study was to determine the effects of serum storage temperature prior to separation from the clot on the levels of potassium in serum. Specimens from at least 4 individuals were used for each analyte. After centrifugation, specimens were refrigerated at 4 degrees C until experimental storage and analysis.
Summary of Findings:
When serum was stored at 32 degrees C on the clot, potassium levels were significantly decreased after 3 (<10%) and 6 h (>10%), but were significantly elevated in specimens stored for 24 h on the clot (approximately 30%). When unseparated serum was stored at room temperature, small increases (<10%) were found in serum stored for 3, 6 and 12 h.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Electrolyte/Metal Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration < 30 min
3 h
6 h
24 h
Storage Storage temperature 32 degrees C
Room temperature
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated
-
Study Purpose
The purpose of this study was to determine the effects of tube fill volume during pneumatic tube transport of blood on the levels of LD in refrigerated serum.
Summary of Findings:
Completely filling collection tubes prior to transport by pneumatic tube system (PTS) resulted in LD levels changing by -1.0 to 13.9% compared to untransported specimens, while LD levels increased by 8.6-30.7% when transported in half-filled tubes.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Protein Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Within hospital transportation method Not transported
Pneumatic tube system
Storage Specimen transport duration/condition Half-full tube
Full tube