Development and validation of sensitive method for determination of serum cotinine in smokers and nonsmokers by liquid chromatography/atmospheric pressure ionization tandem mass spectrometry.
Author(s): Bernert JT Jr, Turner WE, Pirkle JL, Sosnoff CS, Akins JR, Waldrep MK, Ann Q, Covey TR, Whitfield WE, Gunter EW, Miller BB, Patterson DG Jr, Needham LL, Hannon WH, Sampson EJ
Publication: Clin Chem, 1997, Vol. 43, Page 2281-91
PubMed ID: 9439445 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to determine the effects of storage on cotinine concentrations in serum.
Conclusion of Paper
Cotinine was stable in serum stored for 6 weeks at room temperature or 37 degrees C, regardless of concentration. The authors report that cotinine concentrations in serum did not change after more than 4 years at -60 degrees C.
Studies
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Study Purpose
The purpose of this study was to determine the effects of storage on cotinine concentrations in serum.
Summary of Findings:
Cotinine was stable in serum stored for 6 weeks at room temperature or 37 degrees C, regardless of concentration. The authors report that cotinine concentrations in serum did not change after more than 4 years at -60 degrees C.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Small molecule LC-MS or LC-MS/MS Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage temperature -60 degrees C
Room temperature
37 degrees C
Storage Storage duration 0 days
3 days
1 week
2 weeks
4 weeks
6 weeks
> 4 years
Preaquisition Biomarker level Low cotinine concentration
High cotinine concentration