Stability of plasma lactate in vitro in the presence of antiglycolytic agents.
Author(s): Astles R, Williams CP, Sedor F
Publication: Clin Chem, 1994, Vol. 40, Page 1327-30
PubMed ID: 8013108 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of collecting blood with F/Ox, and storage duration and temperature on lactate levels in patients with lactic acidosis and volunteers.
Summary of Findings:
Plasma lactate levels increased by an average of 6.1% when whole blood was collected with F/Ox and stored for 24 h prior to separation of plasma. When patient or volunteer blood was obtained with EDTA or heparin instead of F/Ox, much larger increases in lactate levels were noted after 1 and 8 h of storage on ice or at room temperature. The lactate levels rose faster with storage at room temperature rather than on ice. The authors note that for F/Ox, but not EDTA or heparin, most of the changes in lactate levels occurred within the first hour of storage. As expected, in normal volunteers, exercise increased lactate levels. F alone was found to stabilize lactate concentrations in whole blood as well as F/Ox, while Ox alone decreased lactate levels compared to heparinized blood.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
- Other diagnoses
Platform:
Analyte Technology Platform Small molecule Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 h
1 h
8 h
24 h
48 h
Storage Storage temperature Room temperature
On ice
Biospecimen Acquisition Anticoagulant Sodium fluoride/potassium oxalate
EDTA
Lithium heparin
Preaquisition Diagnosis/ patient condition Lactic acidosis
Normal volunteer
Biospecimen Acquisition Time of biospecimen collection Without exercise
After exercise
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated