Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Rapid HPLC determination of total homocysteine and other thiols in serum and plasma: sex differences and correlation with cobalamin and folate concentrations in healthy subjects.

Author(s): Jacobsen DW, Gatautis VJ, Green R, Robinson K, Savon SR, Secic M, Ji J, Otto JM, Taylor LM Jr

Publication: Clin Chem, 1994, Vol. 40, Page 873-81

PubMed ID: 8087981 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of gender, blood component and detection method on homocysteine, cysteine, CysGly levels in healthy individuals. After separation, serum and EDTA plasma were stored at -20 degrees C until use. Specimens were stored at room temperature in the autosampler for up to 24 h prior to assay.

   Summary of Findings:

   The homocysteine levels measured using a fluorescent detection method were highly correlated with levels detected using electrochemical detection (r=0.974) with no bias. Homocysteine levels were significantly higher in serum than in plasma (p<0.001) and in men compared to women (p=0.002 for both plasma and serum). In men and women there was a negative correlation between serum cobalamin and serum homocysteine (r=-0.423, p=0.0102 and r=-0.400, p=0.0174), and serum cobalamin was negatively correlated with plasma homocysteine in men (r=-0.499, p=0.0019). While plasma cysteine levels were not significantly different in men and women, serum cysteine levels were higher in men than in women (p<0.001). In women, the serum levels of cysteine were significantly higher than plasma levels (p<0.001). Men had higher CysGly levels in plasma and serum than women (both p<0.001). Serum levels of CysGly were higher than plasma levels in men and women (p<0.001 and p=0.022, respectively).

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	HPLC
   Small molecule	Radioassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Preaquisition	Patient gender	Female Male
   HPLC Specific	Detection method	Electrochemical detection Fluorescent detection

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