Interference in the Allegro immunoassay system when blood is collected in silicone-coated tubes.
Author(s): Wickus GG, Mordan RJ, Mathews EA
Publication: Clin Chem, 1992, Vol. 38, Page 2347-8
PubMed ID: 1424143 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of collection container on the measurement of TSH, hCG, prolactin and parathyroid hormone in serum using the Allegro IRMA kits.
Summary of Findings:
Specimens collected from a hypothyroid infant in a microtainer SST had significantly higher TSH levels than dried capillary blood specimens when measured using the Allegro IRMA. Further, when spiked serum was mixed with specimens collected into normal or "no additive" microtainers, Samplette glass tubes, or Monoject glass tubes, only 12-70% of the TSH was measured using the Allegro kit. Similar tube based interference with Allegro assays occurred with the hCG and prolactin assays, but not the parathyroid assay, which does not use avidin-biotin separation. The interfering agent in the microtainer was successfully removed by washing the tube once with distilled water. The authors conclude that water soluble silicone polymers used to coat the interior of blood collection tubes can interfere with the avidin-biotin binding step used in many of the Allegro IRMA kits.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
- Other diagnoses
Platform:
Analyte Technology Platform Peptide Immunoradiometric assay Protein Immunoradiometric assay Glycoprotein Immunoradiometric assay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Diagnosis/ patient condition Hypothyroid
Preaquisition Patient age Newborn
Adult
Biospecimen Acquisition Type of collection container/solution Microtainer SST
"No additive" microtainer
Dried capillary blood
Glass vacutainer
Samplette glass tubes
Monoject glass tubes