Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Effect of in vitro hemolysis on 25 common biochemical tests.

Author(s): Yücel D, Dalva K

Publication: Clin Chem, 1992, Vol. 38, Page 575-7

PubMed ID: 1568325 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of hemolysis on measured levels of 25 clinical chemistry analytes in serum from hospitalized patients. Moderately and severely hemolyzed serum was created by stirring aliquots with a metallic bar for 1 and 2 min, respectively prior to centrifugation.

   Summary of Findings:

   The mean hemoglobin concentrations of non-hemolyzed, moderately hemolyzed, and severely hemolyzed serum were 0.744, 6.743 and 17.608, respectively. Measured levels of lactate dehydrogenase, potassium, acid phosphatase, and prostatic acid phosphatase were increased in moderately hemolyzed serum and severely hemolyzed serum compared to non-hemolyzed serum (all p<0.05 and p<0.001, respectively). Further, cholesterol levels were increased in severely hemolyzed serum compared to non-hemolyzed serum. Non-significant decreased glucose, uric acid, alkaline phosphatase, lipase, and bilirubin (total and direct) levels and increased aspartate aminotransferase, alanine aminotransferase, high-density lipoprotein cholesterol, triglycerides, and inorganic phosphate levels were found in hemolyzed serum compared to non-hemolyzed serum. Correlations were found between the changes in hemoglobin levels in serum and the changes in lactate dehydrogenase (r = 0.789), potassium (r = 0.774), acid phosphatase (r = 0.627), prostatic acid phosphatase (r = 0.539) aspartate aminotransferase (r = 0.543), uric acid (r = 0.374), glucose (r = 0.373), cholesterol (r = 0.340), alanine aminotransferase (r = 0.249), inorganic phosphate (r = 0.213), and lipase (r = 0.202). There were no effects of hemolysis on total protein, albumin, urea, creatinine, sodium, chloride, calcium, magnesium, or amylase levels.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Carbohydrate	Clinical chemistry/auto analyzer
   Protein	Spectrophotometry
   Protein	Clinical chemistry/auto analyzer
   Lipid	Clinical chemistry/auto analyzer
   Steroid	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Hemolysis	Metallic bar stirring-induced Not induced

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