Sample evaporation and its impact on the operating performance of an automated selective-access analytical system.

Author(s): Burtis CA

Publication: Clin Chem, 1990, Vol. 36, Page 544-6

PubMed ID: 2311231 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of sample cup size, specimen volume, capping sample cups, and storage time on the measurement of glucose in serum by the Dimension auto analyzer. Pooled serum from an unspecified origin was used in these studies.

   Summary of Findings:

   Glucose concentration increased with storage duration (6.3-16.5 mg/L increases after 4 h) when serum was held in sample cups prior to analysis in the Dimension auto analyzer. Glucose concentration increased more in aliquots with a smaller volume. Capping the sample cups attenuated these increases in glucose concentration (2.3-13.1 mg/L increases after 4 h), especially for serum held in 2.0 mL cups rather than in 0.5 mL.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Carbohydrate	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Aliquot size/volume	0.125 mL 0.250 mL 0.375 mL 0.5 mL 1.0 mL 1.5 mL 2.0 mL
   Storage	Type of storage container	0.5 mL sample cup 2.0 mL sample cup
   Storage	Storage conditions	Capped Not capped
   Storage	Time at room temperature	0 h 2 h 4 h 6 h 8 h 10 h

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