Monoject Samplette capillary blood container with serum separator evaluated for collection of specimens for therapeutic drug assays and common clinical-chemical tests.
Author(s): Landt M, Norling LL, Steelman M, Smith CH
Publication: Clin Chem, 1986, Vol. 32, Page 523-6
PubMed ID: 3948399 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of collecting and storing blood in Monoject Samplette capillary tubes on measured levels of clinical chemistry analytes and therapeutic drugs in serum.
Conclusion of Paper
When pediatric blood specimens were collected in Samplette tubes, there were higher measured levels of carbon dioxide, theophylline, phenytoin, gentamicin and digoxin, lower levels of chloride, less hemolysis, and less serum yield than when specimens were collected in Caraway tubes. Phenobarbital concentrations decreased with storage of serum in Samplette tubes for more than 8 h, but no other therapeutic drug levels were affected. The authors report no affect of specimen volume on the measured concentration of therapeutic drugs.
Studies
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Study Purpose
The purpose of this study was to determine the effects of collection tube type on the measurement of clinical chemistry analytes and therapeutic drugs in sera.
Summary of Findings:
When pediatric blood specimens were collected in Samplette tubes, a significantly, but clinically irrelevant lower level of chloride was measured compared to specimens collected in Caraway tubes. Specimens collected in Samplette tubes also retained CO2 better then specimens collected in Caraway tubes. Higher concentrations of theophylline, phenytoin, gentamicin and digoxin were measured in specimens collected in Samplette tubes compared to specimens collected in Caraway tubes, but the authors report that only the effect on digoxin was likely to be clinically relevant. Collection of blood in Samplette tubes resulted in less hemolysis and less serum yield than when specimens were collected in Caraway tubes. There was no effect of collection tube type on measured concentrations of sodium, potassium, glucose, urea, creatinine, bilirubin or phenobarbital.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Gas Clinical chemistry/auto analyzer Electrolyte/Metal Clinical chemistry/auto analyzer Carbohydrate Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Small molecule Radioimmunoassay Morphology Light microscopy Morphology Macroscopic observation Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Type of collection container/solution Monoject Samplette capillary serum separator tubes
Caraway tubes
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Study Purpose
The purpose of this study was to determine the effects of specimen volume and storing serum in Samplette tubes on the measurement of therapeutic drugs. Venous blood from adult volunteers was spiked with therapeutic drugs before centrifugation and storage.
Summary of Findings:
There was no effect of storing serum in Samplette tubes (with separator material) for up to 24 h on measured theophylline, phenytoin, and gentamicin concentrations, but phenobarbital concentrations decreased 6-20% after storage of serum for 24 h. The authors report this change in phenobarbital is unlikely to affect clinical decisions, but they recommend analysis within 8 h. The authors also report that specimen volume had no effect on the measurement of any therapeutic drug.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Small molecule Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 8 h
24 h
Biospecimen Aliquots and Components Aliquot size/volume 200-700 ul