Facilitated determination of ionized calcium.

Author(s): Urban P, Buchmann B, Scheidegger D

Publication: Clin Chem, 1985, Vol. 31, Page 264-6

PubMed ID: 3967359 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of heparin brand and storage on Ca levels in whole blood, serum and plasma.

   Summary of Findings:

   There were no differences between the mean concentrations of Ca in whole blood anticoagulated with heparin and serum. When Ca free heparin was used as an anticoagulant, mean Ca concentrations in plasma were more than 20% lower than in serum and decreased with higher concentrations of anticoagulant. Significant, but clinically irrelevant differences were found between brands of Ca-free heparin. Compared to specimens analyzed immediately, a slight increase in whole blood Ca was found when the specimens were stored at room temperature for 2 h, but not when stored on ice.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal
   • Not specified

   Platform:

   Analyte	Technology Platform
   Electrolyte/Metal	Ion selective electrode

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Blood and blood products	Serum Whole blood Plasma
   Biospecimen Acquisition	Anticoagulant	Heparin Calcium-free heparin
   Storage	Storage duration	0 h 2 h
   Storage	Storage temperature	On ice Room temperaure

   View more