Serum-constituents analyses: effect of duration and temperature of storage of clotted blood.

Author(s): Ono T, Kitaguchi K, Takehara M, Shiiba M, Hayami K

Publication: Clin Chem, 1981, Vol. 27, Page 35-8

PubMed ID: 7449120 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of duration and temperature of storage of whole blood prior to centrifugation on the levels of 23 clinical chemistry analytes and turbidity in serum. Blood was collected from 10 fasting women and aliquoted before storage. After experimental storage, blood was centrifuged, and serum was stored at -20 degrees C until analysis. The within day coefficient of variance (CV) for all assays using a pooled specimen was <2.5%.

   Summary of Findings:

   Clinically and statistically significant changes in total protein, aspartate aminotransferase, alanine aminotransferase, LDH, glucose, sodium, potassium, calcium, glucose and phosphorous levels were observed with storage of whole blood prior to centrifugation, but the direction of the changes as well as the storage temperatures and durations causing significant changes were analyte specific. The authors point out that the increase in turbidity of serum after storage of blood at 23 or 30 degrees C did not affect the measurement of serum constituents. Storage of whole blood had no effect on the levels of total bilirubin, direct bilirubin, cholinesterase, urea nitrogen, albumin, zinc sulfate turbidity, thymol turbidity, alkaline phosphatase, leucine aminopeptidase, gamma-glutamyltransferase, amylase, total cholesterol, triglycerides, beta-lipoprotein, creatinine, hemoglobin or uric acid in serum.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	Clinical chemistry/auto analyzer
   Protein	Clinical chemistry/auto analyzer
   Steroid	Clinical chemistry/auto analyzer
   Lipid	Clinical chemistry/auto analyzer
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Protein	Spectrophotometry
   Morphology	Spectrophotometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	<20 min 2 h 4 h 6 h 8 h 24 h 48 h
   Storage	Storage temperature	4 degrees C 23 degrees C 30 degrees C
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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