Importance of blood-collection tubes in plasma lidocaine determinations.

Author(s): Stargel WW, Roe CR, Routledge PA, Shand DG

Publication: Clin Chem, 1979, Vol. 25, Page 617-9

PubMed ID: 380842 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of blood or plasma contact with collection tube stoppers on lidocaine concentration. Clinical specimens and lidocaine spiked specimens from a normal volunteer were collected in plastic syringes and whole blood or plasma was then distributed to the various tubes.

   Summary of Findings:

   In 25 clinical specimens, contact of whole blood with the red stoppers on Vacutainer tubes resulted in a decrease in mean lidocaine concentration in serum from 6.5 ug/mL to 4.9 ug/mL (p<0.001). Similarly, there was a significant decrease in measured lidocaine when whole blood, but not plasma, was added to red or green Vacutainer or red Monoject tubes containing exogenous lidocaine. The effect of using plastic Vacutainer tubes versus an all glass system decreased as lidocaine concentrations increased. However, in plastic Vacutainer tubes or an all glass system, increasing lidocaine concentration decreased the ratio of lidocaine in blood to lidocaine in plasma. Finally, when blood was collected in Vacutainer tubes, lidocaine binding of plasma was 28%, versus 56% when blood was collected in glass.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified
   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	Immunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Type of storage container	Green Vacutainer Red Vacutainer Red Monoject All glass system
   Storage	Storage conditions	Without stopper In contact with stopper or with inversion
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum Whole blood
   Preaquisition	Biomarker level	2 ug/mL lidocaine 4 ug/mL lidocaine 6 ug/mL lidocaine 8 ug/mL lidocaine 10 ug/mL lidocaine 12 ug/mL lidocaine

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