Effect of light and temperature on the stability of creatine kinase in human sera and controls.

Author(s): Perry B, Doumas B, Jendrzejczak B

Publication: Clin Chem, 1979, Vol. 25, Page 625-8

PubMed ID: 466772 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of light exposure, storage duration, storage temperature (-22, 4 or 24 degrees C), and the effect of adding DTT prior to analysis on CK activity in serum. No details of the acquisition or origin of the serum specimens used were provided.

   Summary of Findings:

   Light exposure during room temperature storage had little to no effect on CK levels, nor did up to 24 h storage at -22, 4 or 24 degrees C. Only the specimen with the lowest initial CK levels (53 units/L) showed a decrease of more than 5% (15.1%) with storage for 24 h at 24 degrees C, and this decrease was observed regardless of light exposure. When serum specimens were incubated with DTT prior to measurement, the CK activity increased by an average of 10% within 5 min, regardless of initial CK levels.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	-22 degrees C 4 degrees C 24 degrees C
   Storage	Storage duration	0 h 2 h 6 h 24 h
   Clinical chemistry/auto analyzer Specific	Reaction solution	DTT for 5 min DTT for 20 min No DTT
   Storage	Storage conditions	Exposed to light Protected from light
   Preaquisition	Biomarker level	20-645500 units/L CK

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