Effect of hemolysis on the apparent concentration of insulin in plasma.
Author(s): Cantrell JW, Hochholzer JM, Frings CS
Publication: Clin Chem, 1972, Vol. 18, Page 1423-5
PubMed ID: 4631590 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of anticoagulant type and using serum rather than plasma to measure levels of insulin and hemoglobin.
Conclusion of Paper
Insulin levels were lower, and hemoglobin levels were higher in oxalate-fluoride plasma than in serum. Further, higher hemoglobin levels and lower insulin levels were found in oxalate-fluoride plasma than EDTA plasma.
Studies
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Study Purpose
The purpose of this study was to determine the effects of anticoagulant type and using serum rather than plasma to measure levels of insulin and hemoglobin. The authors report 36 comparisons of values between oxalate-fluoride plasma and serum or EDTA plasma, but they do not specify the number of comparisons for each. Plasma and serum were stored at -20°C.
Summary of Findings:
Insulin levels were lower, and hemoglobin levels were higher in oxalate-fluoride plasma than in serum. Further, 44% higher hemoglobin levels and 27% lower insulin levels were found in oxalate-fluoride plasma than EDTA plasma.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Protein Hematology/ auto analyzer Protein Radioimmunoassay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Blood and blood products Plasma
Serum
Biospecimen Acquisition Anticoagulant Sodium EDTA
Sodium fluoride/potassium oxalate