Stability of plasma enzymes during storage.

Author(s): Juul P

Publication: Clin Chem, 1967, Vol. 13, Page 416-22

PubMed ID: 6024052 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage and anticoagulant on enzyme stability in whole blood, plasma and serum, stored with or without the clot, from healthy individuals. Only 1-2 specimens were examined under each condition.

   Summary of Findings:

   Acetylcholinesterase (AChE) was stable in citrated or heparinated whole blood at 4 degrees C for at least 7 days. Pseudocholinesterase (ChE) was relatively stable in whole blood under all storage conditions, in serum with the clot at -20, 4, or 22 degrees C, or in citrated plasma at 4 degrees C, but ChE levels declined in citrated plasma stored at 38 degrees C. Both glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) were stable in citrated plasma and whole blood stored at 4 degrees C and in serum without the clot stored at -20, 4 and 22 degrees C, but GOT and GPT levels declined in citrated plasma stored at 38 degrees C and increased in heparinated whole blood stored at 4 degrees C for more than a week and in serum stored on the clot at 22 degrees C. LDH was stable in heparinated plasma at 4 degrees C but decreased in citrated plasma stored at 4 degrees C and increased in citrated or heparinated whole blood stored at 4 degrees C or serum stored on the clot at 22 degrees C. Acid phosphatase (Acid Ph) was stable at -20 degrees C but increased when in contact with erythrocytes and decreased when stored at temperatures above -20 degrees C. Alkaline phosphatase (Alk Ph) decreased with storage of whole blood and plasma but was stable in serum at all temperatures examined. Amylase was stable under all temperatures and conditions.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Enzyme assay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 days 1 days 7 days 8 days 30 days 60 days 120 days
   Storage	Storage temperature	-20 degrees C 4 degrees C 22 degrees C 38 degrees C
   Storage	Storage conditions	On the clot Without clot
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum Whole blood
   Biospecimen Acquisition	Anticoagulant	Citrate Heparin None

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