Stability of catecholamines in whole blood: influence of time between collection and centrifugation.
Author(s): Despinasse Q, Choisnard L, Faure P, Guicherd D, Peyrin E, Ravelet C, Chovelon B
Publication: Clin Chem Lab Med, 2020, Vol. , Page
PubMed ID: 33027041 PubMed Review Paper? No
Purpose of Paper
This paper investigated the effects of storing blood on ice for up to 3 h before centrifugation on adrenalin and noradrenalin levels in plasma.
Conclusion of Paper
Adrenaline and noradrenalin levels in plasma were not statistically or clinically affected by storage of blood on ice for ≤3 h before centrifugation.
Studies
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Study Purpose
This study investigated the effects of storing blood on ice for up to 3 h before centrifugation on adrenalin and noradrenalin levels in plasma. Blood was collected into five lithium heparin tubes from 20 hospital staff members. One tube from each volunteer was stored on ice for 0 min, 30 min, 1 h, 2 h, or 3 h before centrifugation at 2000 x g at 4°C for 10 min. Plasma was aliquoted and stored at -80°C until analysis. Plasma was thawed and catecholamine levels for each volunteer were determined by high performance liquid chromatography (HPLC) with a coulometric detection in a single run. Changes were compared to calculated reference change values (RCV) and total change limit (TCL) based on the coefficients of variance.
Summary of Findings:
No significant change in the levels of adrenalin or noradrenalin were observed when centrifugation of blood was delayed by as much as three hours. Further, no clinically relevant changes in adrenalin or noradrenaline levels were observed as all differences were much lower than the RCV and TCL.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Small molecule HPLC Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated
Storage Storage duration 0 min
30 min
1 h
2 h
3 h