Influence of temperature of transport of whole blood on plasma Cu, I, Mn, Se and Zn and Mg concentrations in erythrocytes.
Author(s): Chovelon B, Arnaud J
Publication: Clin Chem Lab Med, 2019, Vol. 57, Page e131-e133
PubMed ID: 30367786 PubMed Review Paper? No
Purpose of Paper
This paper investigated the effects of storing blood at three different temperatures before centrifugation on the levels of copper (Cu), iodine (I), selenium (Se), zinc (Zn), and manganese (Mn) in plasma and levels of magnesium (Mg) in erythrocytes.
Conclusion of Paper
Although significant effects of storage on plasma levels of Cu, I, Se, and Zn and erythrocyte levels of Mg were found, only the change in Zn with storage at room temperature exceeded the total change limit and none of the changes exceeded the reference change value. The authors conclude it is preferable to transport blood on ice for determination of Zn.
Studies
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Study Purpose
This study investigated the effects of storing blood at three different temperatures before centrifugation on the levels of Cu, I, Se, Zn and Mn in plasma and Mg levels in erythrocytes. Blood was collected from 10-13 healthy volunteers on three different days (not specified if different volunteers or the same) into trace element-free Vacutainer tubes containing sodium heparin. Each blood specimen was immediately aliquoted into four specimens. Aliquots were immediately centrifuged at 2000 x g for 10 min or stored at room temperature (mean 22.0˚C, range 21.0-26.5˚C), in the refrigerator (mean 4.29˚C, range 3.60-7.20˚C), or in an oven (mean 35.8˚C, range 33.2-37.3˚C) for 3 h before centrifugation. After centrifugation, plasma and erythrocytes were placed in polypropylene tubes and stored frozen at -19.5˚C until analysis. Levels of Cu, I, Mn, Se, and Zn in plasma were determined by quadrupole inductively coupled plasma mass spectrometry. Mg levels in erythrocytes were determined by flame atomic absorption spectrometry.
Summary of Findings:
ANOVA identified significant effects of storage on plasma levels of Cu (P=0.02), I (P<0.0001), Se (P=0.01), and Zn (P<0.0001) but no effect on Mn in plasma or Mg in erythrocytes. Paired T-test found that Cu and Se were found to increase significantly (0.83% and 2.11%, respectively) when stored for 3 h in the oven (mean 35.8˚C) compared to specimens processed immediately. I and Zn were found to increase significantly (4.37-5.85% and 1.57-7.48%, respectively) when stored at any of the tested temperatures and, for the majority of specimens, the largest increase was found when stored at room temperature (30 of 33 for Zn and 11 of 30 for I). While ANOVA did not find a significant effect of storage on Mg levels in erythrocytes, a significant decrease was observed after storage at room temperature (-1.81%) and in the oven ( -2.18%). However, only the change in Zn with storage at room temperature exceeded the total change limit and none of the changes exceeded the reference change value. The authors conclude it is preferable to transport blood on ice for determination of Zn.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Electrolyte/Metal Inductively coupled plasma mass spectrometry Electrolyte/Metal Flame atomic absorption spectrometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated
Storage Storage temperature Room temperature (mean 22.0˚C)
Refrigerator (mean 4.29˚C)
Oven (mean 35.8˚C)
Storage Storage duration 0 h
3 h